Journal of Acupuncture and Meridian Studies
Volume 2, Issue 1 , Pages 66-70, March 2009

Visualizing the Network of Bonghan Ducts in the Omentum and Peritoneum by Using Trypan Blue

  • Byung-Cheon Lee

      Affiliations

    • Research Institute of Basic Sciences, Seoul National University, Seoul, Korea
    • Research Division of the Korean Pharmacopunture Institute, Seoul, Korea
    • ,
  • Ki Woo Kim

      Affiliations

    • National Instrumentation Center for Environmental Management, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea
    • ,
  • Kwang-Sup Soh

      Affiliations

    • Biomedical Physics Laboratory, School of Physics and Astronomy, Seoul National University, Seoul, Korea
    • Corresponding Author InformationCorresponding author. Biomedical Physics Laboratory, School of Physics and Astronomy, Seoul National University, Seoul 151–747, Korea

Received 24 December 2008; accepted 14 January 2009.

Abstract 

A visualizing agent, Trypan blue, was found to be preferentially effective for Bonghan ducts (BHDs) and corpuscles compared to blood vessels or adipose tissues. By using it, we observed a weblike network of BHDs which was in various membrane structures, such as the peritoneum, and omenta. This network of BHDs in the membrane structures was connected to the freely movable BHDs which did not adhere to the surfaces or wrapping membranes of internal organs. In addition, tracing BHDs in adipose tissues became possible because Trypan blue does not stain adipose tissue.

Key Words:  acupuncture , adipose tissue , Bonghan corpuscle , Bonghan duct , omentum , peritoneum , Trypan blue

 

PII: S2005-2901(09)60017-0

doi:10.1016/S2005-2901(09)60017-0

Journal of Acupuncture and Meridian Studies
Volume 2, Issue 1 , Pages 66-70, March 2009

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