Proteomic Analysis for Tissues and Liquid from Bonghan Ducts on Rabbit Intestinal Surfaces

A glass capillary was inserted into a Bonghan duct, held by microforceps, above the large intestine of rabbit using in situ and in vivo stereomicroscopy (SZX12, Olympus, Japan). The capillary tip (dotted circle) was correctly inserted into the Bonghan duct in order to extract its liquid. The scale bar, located in the bottom right, is 5 mm.

Protein conversion using the blast similarity operation. The process consisted of three steps: (1) obtaining the amino acid sequence of a given rabbit protein; (2) running the blast similarity operation; and (3) selecting the corresponding human protein that exhibited the lowest E-value. For the blast similarity operation, the human ‘RefSeq protein’ was used as the database for blast searching and ‘BLASTP’ was applied to compare protein sequences. The protein with the lowest E-value was selected as the best match. If two or more proteins with the same lowest E-value were identified, the upper listed protein was arbitrarily chosen.

Clustering of proteins identified in Bonghan liquid. The clustering was executed consistent with the three GO domains of biological processes (A), molecular functions (B) and cellular compartmentalization (C). The pie chart values indicate the numbers of proteins included in each category. Certain proteins belonged to more than two categories and as a result the total number of proteins in the pie chart is different from the number of proteins identified in Table 1.

Clustering of proteins identified in Bonghan duct. Note the similarity with Figure 3.