Journal of Acupuncture and Meridian Studies
Volume 1, Issue 2 , Pages 143-148 , December 2008

Treatment of Mycoplasma wenyonii Infection in Cows with Imidocarb Dipropionate Injection-acupuncture

  • Zhengui Yan

      Affiliations

    • College of Veterinary Medicine and Animal Science, Shandong Agricultural University, Tai'an, China
    • ,
  • Jianzhu Liu

      Affiliations

    • College of Veterinary Medicine and Animal Science, Shandong Agricultural University, Tai'an, China
    • Corresponding Author InformationCorresponding author. College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China
    • ,
  • Tiantian Chen

      Affiliations

    • College of Veterinary Medicine and Animal Science, Shandong Agricultural University, Tai'an, China
    • ,
  • Ziqiang Cheng

      Affiliations

    • College of Veterinary Medicine and Animal Science, Shandong Agricultural University, Tai'an, China
    • ,
  • Huijun Guo

      Affiliations

    • College of Veterinary Medicine and Animal Science, Shandong Agricultural University, Tai'an, China
    • ,
  • Zhenyong Wang

      Affiliations

    • College of Veterinary Medicine and Animal Science, Shandong Agricultural University, Tai'an, China
    • ,
  • Yong Wang

      Affiliations

    • College of Animal Science and Technology, Tarim University, Alar, Xinjiang Uygur Autonomous Region, China

Received 31 March 2008 ,Accepted 3 July 2008.

  • Image Result

    Light microscopic examination of blood smears from shorthorn cows. Numerous extracellular parasites, consistent with M. wenyonii, were observed both on the surface of erythrocytes and in the plasma sp

    Light microscopic examination of blood smears from shorthorn cows. Numerous extracellular parasites, consistent with M. wenyonii, were observed both on the surface of erythrocytes and in the plasma space (arrows). The organisms were small, basophilic, coccus-shaped and ring-shaped, and affected nearly 100% of the erythrocytes.

  • Image Result
    PCR based detection of M. wenyonii 16S rRNA in shorthorn cows. The amplified 341 bp product, from the positive sample control (line 1) and positive test samples (lines 2-4) were subjected to electroph

    PCR based detection of M. wenyonii 16S rRNA in shorthorn cows. The amplified 341 bp product, from the positive sample control (line 1) and positive test samples (lines 2-4) were subjected to electrophoresis in 1.2%-agarose gel and stained with ethidium bromide. Marker 2000 (M) and a negative control free of DNA template (line 5) were run in parallel.

PII: S2005-2901(09)60035-2

doi: 10.1016/S2005-2901(09)60035-2

Journal of Acupuncture and Meridian Studies
Volume 1, Issue 2 , Pages 143-148 , December 2008

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