Journal of Acupuncture and Meridian Studies
Volume 2, Issue 4 , Pages 301-305 , December 2009

DiI Staining of Fine Branches of Bonghan Ducts on Surface of Rat Abdominal Organs

  • Byung-Cheon Lee

      Affiliations

    • Biomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University, Seoul, Korea
    • Research Division, Korean Pharmacoacupuncture Institute, Seoul, Korea
    • ,
  • Seong-Uk Jhang

      Affiliations

    • Biomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University, Seoul, Korea
    • ,
  • Jae-Hong Choi

      Affiliations

    • Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Seoul National University, Seoul, Korea
    • ,
  • So-Yeong Lee

      Affiliations

    • Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Seoul National University, Seoul, Korea
    • ,
  • Pan-Dong Ryu

      Affiliations

    • Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Seoul National University, Seoul, Korea
    • Corresponding Author InformationCorresponding author. Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Seoul National University, Seoul, 151-742 Korea
    • ,
  • Kwang-Sup Soh

      Affiliations

    • Biomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University, Seoul, Korea

References 

  1. Kim BH . On the Kyungrak System . J Acad Sci DPR Korea . 1963;1–35
  2. Shin HS , Johng HM , Lee BC , Cho SI , Soh KS , Baik KY , et al.   Feulgen reaction study of novel threadlike structures (Bonghan ducts) on the surfaces of mammalian organs . Anat Rec B New Anat . 2005;284:35–40
  3. Lee BC , Yoo JS , Ogay V , Kim KW , Dobberstein H , Soh KS , et al.   Electron microscopic study of novel threadlike structures on the surfaces of mammalian organs . Microsc Res Tech . 2007;70:34–43
  4. Hong S , Yoo JS , Hong JY , Lee BC , Soh KS , Lee SH , et al.   Immunohistochemical and electron microscopic study of the meridian-like system on the surface of internal organs of rats . Acupunct Electrother Res . 2007;32:195–210
  5. Sung B , Kim MS , Lee BC , Yoo JS , Lee SH , Kim YJ , et al.   Measurement of flow speed in the channels of novel threadlike structures on the surfaces of mammalian organs . Naturwissenschaften . 2008;95:117–124
  6. Lee BC , Baik KY , Johng HM , Nam TJ , Lee J , Sung B , et al.   Acridine orange staining method to reveal the characteristic features of an intravascular threadlike structure . Anat Rec B New Anat . 2004;278:27–30
  7. Lee BC , Yoo JS , Baik KY , Sung B , Lee J , Soh KS . Development of a fluorescence stereomicroscope and observation of Bong-Han corpuscles inside blood vessels . Indian J Exp Biol . 2008;46:330–335
  8. Yoo JS , Kim MS , Ogay V , Soh KS . In vivo visualization of bonghan ducts inside blood vessels of mice by using an alcian blue staining method . Indian J Exp Biol . 2008;46:336–339
  9. Lee BC , Yoo JS , Baik KY , Kim KW , Soh KS . Novel threadlike structures (Bonghan ducts) inside lymphatic vessels of rabbits visualized with a Janus Green B staining method . Anat Rec B New Anat . 2005;286:1–7
  10. Lee C , Seol SK , Lee BC , Hong YK , Je JH , Soh KS . Alcian blue staining method to visualize bonghan threads inside large caliber lymphatic vessels and x-ray microtomography to reveal their microchannels . Lymphat Res Biol . 2006;4:181–190
  11. Johng HM , Yoo JS , Yoon TJ , Shin HS , Lee BC , Lee C , et al.   Use of magnetic nanoparticles to visualize threadlike structures inside lymphatic vessels of rats . Evid Based Complement Alternat Med. . 2007;4:77–82
  12. Lee BC , Kim S , Soh KS . Novel anatomic structures in the brain and spinal cord of rabbit that may belong to the Bonghan system of potential acupuncture meridians . J Acupunct Meridian Stud . 2008;1:29–35
  13. Kim BH . Kyungrak System . J Acad Sci DPR Korea . 1965;1–38
  14. Honig MG , Hume RI . Dil and DiO: versatile fluorescent dyes for neuronal labeling and pathway tracing . Trend Neurosci . 1989;12:333–340
  15. Prentø P . A contribution to the theory of biological staining based on the principles for structural organization of biological macromolecules . Biotech Histochem . 2001;76:137–161
  16. Köbbert C , Apps R , Bechmann I , Lanciego JL , Mey J , Thanos S . Current concepts in neuroanatomical tracing . Prog Neurobiol . 2000;62:327–351
  17. Swift MJ , Crago PE , Grill WM . Applied electric fields accelerate the diffusion rate and increase the diffusion distance of DiI in fixed tissue . J Neurosci Methods . 2005;141:155–163
  18. Honig MG , Hume RI . Fluorescent carbocyanine dyes allow living neurons of identified origin to be studied in longterm cultures . J Cell Biol . 1986;103:171–187

PII: S2005-2901(09)60072-8

doi: 10.1016/S2005-2901(09)60072-8

Journal of Acupuncture and Meridian Studies
Volume 2, Issue 4 , Pages 301-305 , December 2009

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