Journal of Acupuncture and Meridian Studies
Volume 3, Issue 1 , Pages 32-37 , March 2010

The Effect of the Major Components of Fructus Cnidii on Osteoblasts In Vitro

  • Wenping Zhang

      Affiliations

    • Department of Acupuncture, Suzuka University of Medical Science, Mie, Japan
    • Corresponding Author InformationCorresponding author. Institute of Traditional Chinese Medicine, Suzuka University of Medical Science, 1275-3 Kishioka, Suzuka, Mie 510-0226, Japan
    • ,
  • Dongming Ma

      Affiliations

    • Tianjin University of Traditional Chinese Medicine, Tianjin, China
    • ,
  • Qiduo Zhao

      Affiliations

    • Tianjin University of Traditional Chinese Medicine, Tianjin, China
    • ,
  • Torao Ishida

      Affiliations

    • Department of Acupuncture, Suzuka University of Medical Science, Mie, Japan
    • Corresponding Author InformationCorresponding author. Institute of Traditional Chinese Medicine, Suzuka University of Medical Science, 1275-3 Kishioka, Suzuka, Mie 510-0226, Japan

Received 8 June 2009 ,Accepted 27 October 2009.

  • Image Result

    (A) Primary cultured osteoblast cells at 3 days (4×). (B) Osteoblast cells of the second passage at 5 days (40×). (C) Hematoxylin-eosin staining (100×). (D) Immunofluorescence staining (200×).

    (A) Primary cultured osteoblast cells at 3 days (4×). (B) Osteoblast cells of the second passage at 5 days (40×). (C) Hematoxylin-eosin staining (100×). (D) Immunofluorescence staining (200×).

  • Image Result

    Effects of osthole at various concentrations on the proliferation of osteoblast cells in vitro, as determined by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide or MTT assay (*p < 0.05 vs

    Effects of osthole at various concentrations on the proliferation of osteoblast cells in vitro, as determined by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide or MTT assay (*p < 0.05 vs. control).

  • Image Result
    Effects of osthole at various concentrations on the differentiation of osteoblasts in vitro, as determined by an alkaline phosphatase (ALP) activity assay (*p < 0.05 vs. control).

    Effects of osthole at various concentrations on the differentiation of osteoblasts in vitro, as determined by an alkaline phosphatase (ALP) activity assay (*p < 0.05 vs. control).

  • Image Result
    Effects of osthole at various concentrations on the formation of mineralized nodules in an osteoblast culture system (*p < 0.05 vs. control).

    Effects of osthole at various concentrations on the formation of mineralized nodules in an osteoblast culture system (*p < 0.05 vs. control).

PII: S2005-2901(10)60005-2

doi: 10.1016/S2005-2901(10)60005-2

Journal of Acupuncture and Meridian Studies
Volume 3, Issue 1 , Pages 32-37 , March 2010

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