Primo-Vessels as New Flow Paths for Intratesticular Injected Dye in Rats

Han, Hyun-Jung; Ogay, Vyacheslav; Park, Sang-Jun; Lee, Byung-Cheon; Kim, Ki-Woo; Lee, Yu Won; Lee, Jin-Kyu; Soh, Kwang-Sup

doi:10.1016/S2005-2901(10)60016-7

« Previous Next »Journal of Acupuncture and Meridian Studies
Volume 3, Issue 2 , Pages 81-88, June 2010

Primo-Vessels as New Flow Paths for Intratesticular Injected Dye in Rats

Hyun-Jung Han
- Biomedical Physics Laboratory, Department of Physics and Astronomy and Center for Theoretical Physics, Seoul National University, Seoul, Korea
Vyacheslav Ogay
- Biomedical Physics Laboratory, Department of Physics and Astronomy and Center for Theoretical Physics, Seoul National University, Seoul, Korea
Sang-Jun Park
- Biomedical Physics Laboratory, Department of Physics and Astronomy and Center for Theoretical Physics, Seoul National University, Seoul, Korea
- Korean Pharmacopuncture Institute, Seoul, Korea
Byung-Cheon Lee
- Biomedical Physics Laboratory, Department of Physics and Astronomy and Center for Theoretical Physics, Seoul National University, Seoul, Korea
- Korean Pharmacopuncture Institute, Seoul, Korea
Ki-Woo Kim
- National Instrumentation Center for Environmental Management, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea
Yu Won Lee
- Materials Chemistry Laboratory, School of Chemistry, Seoul National University, Seoul, Korea
Jin-Kyu Lee
- Materials Chemistry Laboratory, School of Chemistry, Seoul National University, Seoul, Korea
Kwang-Sup Soh
- Biomedical Physics Laboratory, Department of Physics and Astronomy and Center for Theoretical Physics, Seoul National University, Seoul, Korea
- Corresponding author. Biomedical Physics Laboratory, Department of Physics and Astronomy and Center for Theoretical Physics, Seoul National University, Gwanak 599 Gwanak-ro, Gwanak-gu, Seoul 151-747, Korea

Received 4 February 2010; accepted 14 April 2010.

Abstract

After intratesticular injection of a chromium hematoxylin and fluorescent nanoparticle solution, we found a novel flow path in the abdominal cavity consisting of primo-vessels and primo-nodes. This flow path formed a network that crossed over the surfaces of abdominal organs, and generally linked to the greater omentum and adjacent visceral peritoneum. Some of these structures terminated at organs such as the small intestine and the urinary bladder; occasionally, the network entered the parenchyma of organs. The semitransparent primo-vessels and nodes were wholly or partially stained dark-blue by chromium hematoxylin. Injected nanoparticles were also observed in primo-vessels and nodes as well as the parenchyma of organs which were the site of primo-vessel termination. Transmission electron microscopy showed that the primo-vessels consist of many sinuses (4–6 μm), surrounded by collagen fibers, specific granules, cellular remnants, immune cells, extracellular matrices, and hematopoietic cells. These histological features are completely different from blood and lymph vessels indicating that primo-vessels are novel structures that allow the flow of dye.

Key Words: chromium hematoxylin , nanoparticle , novel flow path , primo-vessels , testis